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Hi
We run two confocal microscopes Leica SP5 - inverted and upright - using a Chameleon at NBT of the Italian Institute of Technology.
Cia
Alby

p.s. we can send set up details

On Jul 21, 2011, at 11:36 PM, Armstrong, Brian wrote:

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> 
> We run two Confocal microscopes off of one Chameleon laser. We split the beam with a polarizing beam splitter, the two beams then go to two AOMs (Zeiss) which controls percent power. We operate the Chameleon by USB to its own computer, that way if someone leaves they do not turn off the laser for the other user.  
> We have the entire system on one optical table (8' x 5'), I would not try it on two tables. My understanding is that you do not want to run a 2P beam through a fiber, although I have heard of someone doing it recently, perhaps others can comment about doing this.
> 
> The systems work well and we seem to have plenty of power out of a Chameleon 210 for both microscopes.
> 
> Hope this helps, 
> 
> Brian D Armstrong PhD
> Assistant Research Professor
> Light Microscopy Core 
> Beckman Research Institute
> City of Hope
> Dept of Neuroscience
> 1450 E Duarte Rd
> Duarte, CA 91010
> 626-256-4673 x62872
> 
> http://www.cityofhope.org/research/support/Light-Microscopy-Digital-Imaging/Pages/default.aspx
> 
> 
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Arvydas Matiukas
> Sent: Thursday, July 21, 2011 2:25 PM
> To: [log in to unmask]
> Subject: Driving two 2p scopes with a single 2p laser
> 
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> 
> Dear list,
> 
> I wonder if anybody tried to simultaneously run two 2p systems
> off single Chameleon 2p laser.
> 
> Additional issue is how to deliver 2p beam to the second system
> which we want to setup on a separate optical table. Will a fiber
> connection work? Please suggest any potential options that would be
> significantly cheaper than buying second Chameleon laser ($160k).
> 
> 
> Thanks,
> 
> 
> 
> 
> 
> Arvydas Matiukas, Ph.D.
> Director of Confocal&Two-Photon Core
> Department of Pharmacology
> SUNY Upstate Medical University
> 766 Irving Ave., WH 3167
> Syracuse, NY 13210
> tel.: 315-464-7997
> fax: 315-464-8014
> email: [log in to unmask]
> 
> 
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