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October 2011

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Subject:
Re: photo-switchable reporter
From:
Ricardo Henriques <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 4 Oct 2011 12:39:53 +0200
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Hi Julia,

mEos2 is a photo-convertible genetically encoded fluorophore that switches from green to orange upon UV-light photo-activation, it is extremely popular in the super-resolution field (see the PALM) due to its high contrast and decent photon output. Alternatively, if you can live with fixed cells+ABs and a more stochastic/less controllable photo-switchable fluorophore, then classic Cy5 or Alexa647 will also do an excellent job (see the dSTORM techniques).

Here's a recent review with of some of the current photo-switchable fluorophores:
http://mhlangalab.synbio.csir.co.za/assets/publications/1/original/HenriquesMhlanga2009Reprinted.pdf?1266936429

Cheers,
R

Ricardo Henriques
Instituto de Medicina Molecular (Lisbon, Portugal).
For contact information see: https://sites.google.com/site/paxcalpt/

On Oct 4, 2011, at 12:29 PM, Julia Edgar wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Dear All
> Can anybody recommend a good photo-switchable and/or photo-activatable reporter. 
> Thank you
> Julia Edgar
> University of Glasgow
>

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