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Hi Ricardo:

The non-uniform illuminaiton can be corrected post-acquisition which can be done by the polynomial fitting to each row of the image intensity profile and followed by a subtraction. 


________________________________________
From: Ricardo Henriques [[log in to unmask]]
Sent: Monday, October 03, 2011 8:12 AM
To: [log in to unmask]
Subject: Advice on citations for homogenized fluorescence illumination

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Hello everyone,

I'm preparing a small manuscript that in part covers the concept that most fluorescence microscopes (SD, WF, TIRF, ...) have a non-homogenous illumination of the field of view - typically gaussian-shaped, less intensity on the image extremities. I was wondering if you could advise me on what you consider the most interesting publications tackling or approaching this subject.

Thank you in advanced.
Best regards,
Ricardo Henriques

Ricardo Henriques
Instituto de Medicina Molecular (Lisbon, Portugal).
For contact information see: https://sites.google.com/site/paxcalpt/