CONFOCALMICROSCOPY Archives

February 2012

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Subject:
Re: Labeling sperm
From:
Julian Smith III <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 17 Feb 2012 17:22:39 -0500
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*****
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*****

Hoechst 33342?  I believe I remember a paper on Bryozoan fertilization 
using that.
Julian

On 2/17/12 5:05 PM, Pilger, John wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> I have a need to label the nucleus of some marine invertebrate sperm to
> track the movement of the male pronucleus within the egg. Does anyone
> have a suggestion of a marker that could be incorporated into live sperm
> without interfering with their ability to fertilize the eggs?
>
> Thanks for you help and wisdom.
>
> John
>


-- 
Julian P.S. Smith III
  Director, Winthrop Microscopy Facility
Dept. of Biology
Winthrop University
520 Cherry Rd.
Rock Hill, SC  29733

803-323-2111 x6427 (vox)
803-323-3448 (fax)
803-524-2347 (cell)
Research Website www.birdnest.org/smithj
Personal Website www.rociada-east.net

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