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I'm surprised the NHS ester didn't work well, since it should label all proteins.  In what way did it not work?  Ununiform labeling?  Too dim?

Jason

Jason A. Kilgore
Technical Application Scientist
Molecular Probes Labeling and Detection Technologies
Cells Systems Division
 
T 1 800 955 6288 then option 4, then option 6,  or  541 335 0353 . F 541 335 0238
29851 Willow Creek Rd . Eugene . OR . 97402-9132 . United States
www.invitrogen.com/technicalsupport

 




-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Doreen MILIUS
Sent: Monday, July 16, 2012 7:29 AM
To: [log in to unmask]
Subject: ant surface stainig

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Dear list members,

we are trying to perform an unspecific fluorescence surface staining of whole ants to see the structure of the cuticle. We have tried water-soluble protein binding dyes like NHS-ster Dylight 549 which didn't work so well and are now thinking about trying out hydrophobic dyes that stain membranes unspecifically. Does anyone have experience with staining insect surfaces and could give a suggestion. This would be of great help.

Thanks a lot, Doreen


- - -
Doreen Milius

Bioimaging Facility
Institute of Science and Technology Austria
Am Campus 1
A-3400 Maria Gugging