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On 31.07.2012 19:58, Craig Brideau wrote:
> Multiphoton is just fluorescence with a fancy laser.

There is so much more to multiphoton than just 2P-excited fluorescence!

As Guy already mentioned, starch granules work great with SHG, we once 
did that on potatoes sliced with a microtome :-) Cellulose also should 
give a SHG signal, but we didn't test that.

THG will give you nice patterns on fat droplets in water, e.g. condensed 
milk (or sunscreen, but that's not food, then) and I would assume that 
the same would happen with CARS but that we don't have.

If I were to speculate, I would guess that you probably find some 
relation of the SHG pattern caused by muscle-myosin and the age of meat 
- but that is just that, a speculation.

Steffen



-- 
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Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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