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March 2013

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Confocal Microscopy List <[log in to unmask]>
Date:
Tue, 12 Mar 2013 14:08:16 -0400
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Confocal Microscopy List <[log in to unmask]>
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Re: side-by-side merge
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Jim Haley <[log in to unmask]>
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*****
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Michael,

If you would email me one of the PIC files, I will test it and see if I 
have a solution for you.  I have gotten these to open/convert in the 
past, but there were different versions of the PIC format, so I would 
like to test and experiment before I can give you a definitive solution.

Thanks And Have a Great Day!

Jim Haley

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Jim Haley
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MVIA, Inc.
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On 3/12/2013 10:50 AM, Cammer, Michael wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> But the original BioRad merge feature made each channel only 4 bits and scrambled the LUT in an odd way, so don't rue too much...
>
> If anyone has an importer, converter etc to open these old .pic merged images, please let me know!
>
> Regards,
>
> Michael C.
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Unruh, Jay
> Sent: Tuesday, March 12, 2013 10:41 AM
> To: [log in to unmask]
> Subject: Re: side-by-side merge
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> There are a couple of ways to do this in ImageJ.  First you need to split the colors with Image>Color>Split Channels.  If you want to merge them side by side permanently, convert to RGB (Image>Type>RGB Color) use Image>Stacks>Tools>Combine.  If you just want to browse them together, use Analyze>Tools>Sync Windows.
>
> Jay
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Michal Opas
> Sent: Tuesday, March 12, 2013 8:35 AM
> To: [log in to unmask]
> Subject: side-by-side merge
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear Listers,
>
> This harks back to prhistory:
> on Bio-Rad MRC 600 and Confocal Assistant by Todd Brelje one could merge dual wavelength images taken simultaneously using split screen ("split
> box")  feature of the scope.
> This "side-by-side merge" feature was very convenient for the dual excitation, split image frames as one could quickly merge left with right and see how good the merged image is.
> Sadly, the Confocal Assistant will not work under 64-bit OS.
> I look for a functionality allowing for "side-by-side merge" in ImageJ and Fiji but failed to find it.
> I am discombobulated: is such a useful feature gone forever (past Win
> XP) or my ineptitude is gross?
>
> Thank you very much for advice.
>
> Michal
>
>

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