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Holly,

          I'm assuming you want to stop the exposure to the operator on an inverted microscope.  Is that correct?  Most inverted fluorescence microscopes already come with a plastic safety screen for that purpose - isn't that enough?  If not I'd suggest getting some theatrical plastic filter.  This comes in big sheets, is very cheap, and has guaranteed absorption spectra.  A yellow-orange tint should do the trick, but check the spectra to see that it will block your laser lines.  You can easily cut bits to fit, and replace them as often as necessary.    I can't see that window tint would do much since it's mainly designed to block IR.  

                                                Guy

-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Holly L. AARON
Sent: Wednesday, 20 March 2013 3:30 AM
To: [log in to unmask]
Subject: Not confocal question: Laser Shielding

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Hi, All - I apologize for the non-confocal nature of this question, but I cannot think of a better resource than this community. I am looking for some sort of "tinting" or similar to minimize the laser exposure through a clear plastic incubation chamber on a TIRF-based rig.  Our laser safety officer suggested tinting would be okay, but I have no idea where to look for that in custom sizes/amounts. Would window tinting for vehicles work?
Has anyone done this? Would love to hear your experience.

Thanks as always!
-Holly
___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA  94720-2751
510.642.2901
510.642.5741 fax
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http://imaging.berkeley.edu

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