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March 2013

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Confocal Microscopy List <[log in to unmask]>
Subject:
Blocking in immunocytochemsitry - ImageIT FX Enhancer?
From:
Christophe Leterrier <[log in to unmask]>
Date:
Thu, 21 Mar 2013 10:23:52 +0100
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Hi,

Not a confocal question, but a lot of people are doing immuno here so I
thought I'd ask. We are currently using gelatin as the main blocker in our
imunocytochemistry protocol. I was wondering if we could get better
staining (less background / less non specific labeling) by switching to
something else and found Life Technologie's ImageIT FX Enhancer (
http://products.invitrogen.com/ivgn/product/I36933). Does someone have
experience with this, and is the added cost justified? I try to stay clear
of proprietary formulas but sometimes (see Prolong Gold) a "secret" product
does work better.

Thanks for the advices,

Christophe

--
Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France

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