*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Hi all,

We are keen to try out the Genteal that was mentioned recently.

In Australia there are two formulations sold and neither of the packages look the same as the pictures on Amazon.

There is:-

Genteal Lubricant Eye Drops - <http://www.chemistaustralia.com.au/products/genteal-lubricant-eye-drop-0-3-15ml.html>
and
Genteal Lubricating Eye Gel - <http://www.chemistaustralia.com.au/products/genteal-lubricant-eye-drop-0-3-15ml.html>

From the Novartis site it looks likes both have hypromellose whereas the Gel adds carbomer 980.
<http://www.novartis.com.au/PI_PDF/ged_geg.pdf>


Can the Genteal users please point us in the right direction :)

Thanks,

Adrian Smith,
Centenary Institute, Sydney, Australia


On 20/02/2013, at 2:00 PM, Watkins, Simon C wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> For us genteal gel 
> http://www.amazon.com/GenTeal-Lubricant-Moderate-Severe-Relief/dp/B001GBIS7
> Y/ref=pd_bxgy_hpc_img_z works very well..we have used it overnight in some
> conditions.. Though this was within an humidified chamber
> 
> Simon Watkins Ph.D
> 
> Professor and Vice Chair Cell Biology
> Professor Immunology
> Director Center for Biologic Imaging
> University of Pittsburgh
> Bsts 225 3550 terrace st
> Pittsburgh PA 15261
> Www.cbi.pitt.edu <http://Www.cbi.pitt.edu/>
> 412-352-2277
> 
> 
> 
> 
> 
> 
> On 2/19/13 6:04 PM, "Benjamin Hibbs" <[log in to unmask]> wrote:
> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> Hi Adam,
>> 
>> I'm sure some of the other members of the list have more experience than
>> I. However, perhaps you could try making a continual water source like
>> the leica system. I know Steve Cody has developed some nifty techniques
>> to maintain the immersion water automatically, but even a manual top-up
>> every few hours could help you in conjunction with your reservoir
>> approach.
>> 
>> Best of luck,
>> 
>> Ben
>> 
>> 
>> Ben Hibbs
>> Platform Support Officer‹Advanced Fluorescence Imaging
>> The Melbourne Materials Institute (MMI)
>> University of Melbourne, Victoria 3010, Australia
>> Email: [log in to unmask]<mailto:[log in to unmask]>
>> Phone: +61 (0)3 9035-7749
>> 
>> 
>> 
>> 
>> 
>> 
>> 
>> On 20/02/2013, at 7:01 AM, Adam White
>> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>> 
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>> 
>> I did a search of the archive but I think I have exhausted most of the
>> previous
>> suggestions... We are attempting to do some overnight live imaging
>> experiments using 2PE microscopy.  We are using the Nikon Apo LWD 25x/1.1
>> water immersion objective on an inverted stand and our main problem is
>> keeping the immersion medium in place for longer than a couple of hours.
>> We
>> have tried using the Cargille oil with a 1.335 RI but it does not have
>> enough
>> viscosity/surface tension to be useful.  We have tried using ultrasound
>> gel but
>> this dries out over time and only gives us about 5 hours of images.  I
>> have also
>> tried all manner of different sealants/gloves/o-rings to varying degrees
>> of
>> success/reproducibility.  The best I have found is to use a stretched-out
>> glove
>> finger (plus a sealant) and just fill up the resulting "reservoir" with
>> water.  This
>> has given us enough volume to get about 10 hours worth of imaging but is
>> hard
>> to keep consistent.  Obviously, my question is whether someone has
>> developed
>> or knows of a better system for doing this?  Perhaps a perfusion system
>> or a
>> more durable glove-finger type solution?  I have seen the Leica system
>> but this
>> won't fit our objective...  Any input you have to offer would be most
>> appreciated.
>> 
>> Best,
>> Adam
>> 
>> 
>> Adam B. White, Ph.D.
>> Confocal & Specialized Microscopy Shared Resource
>> Herbert Irving Comprehensive Cancer Center
>> Columbia University
>> 1130 Saint Nicholas Ave, 222A
>> New York, NY 10032