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Does anybody have time to throw some Genteal in a fluorometer and see 
how it behaves throughout the spectrum?
Just curious.

Dan


On 3/3/2013 8:24 PM, Adrian Smith wrote:
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>
> Hi all,
>
> We are keen to try out the Genteal that was mentioned recently.
>
> In Australia there are two formulations sold and neither of the packages look the same as the pictures on Amazon.
>
> There is:-
>
> Genteal Lubricant Eye Drops - <http://www.chemistaustralia.com.au/products/genteal-lubricant-eye-drop-0-3-15ml.html>
> and
> Genteal Lubricating Eye Gel - <http://www.chemistaustralia.com.au/products/genteal-lubricant-eye-drop-0-3-15ml.html>
>
>  From the Novartis site it looks likes both have hypromellose whereas the Gel adds carbomer 980.
> <http://www.novartis.com.au/PI_PDF/ged_geg.pdf>
>
>
> Can the Genteal users please point us in the right direction :)
>
> Thanks,
>
> Adrian Smith,
> Centenary Institute, Sydney, Australia
>
>
> On 20/02/2013, at 2:00 PM, Watkins, Simon C wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> For us genteal gel
>> http://www.amazon.com/GenTeal-Lubricant-Moderate-Severe-Relief/dp/B001GBIS7
>> Y/ref=pd_bxgy_hpc_img_z works very well..we have used it overnight in some
>> conditions.. Though this was within an humidified chamber
>>
>> Simon Watkins Ph.D
>>
>> Professor and Vice Chair Cell Biology
>> Professor Immunology
>> Director Center for Biologic Imaging
>> University of Pittsburgh
>> Bsts 225 3550 terrace st
>> Pittsburgh PA 15261
>> Www.cbi.pitt.edu <http://Www.cbi.pitt.edu/>
>> 412-352-2277
>>
>>
>>
>>
>>
>>
>> On 2/19/13 6:04 PM, "Benjamin Hibbs" <[log in to unmask]> wrote:
>>
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>>>
>>> Hi Adam,
>>>
>>> I'm sure some of the other members of the list have more experience than
>>> I. However, perhaps you could try making a continual water source like
>>> the leica system. I know Steve Cody has developed some nifty techniques
>>> to maintain the immersion water automatically, but even a manual top-up
>>> every few hours could help you in conjunction with your reservoir
>>> approach.
>>>
>>> Best of luck,
>>>
>>> Ben
>>>
>>>
>>> Ben Hibbs
>>> Platform Support Officer‹Advanced Fluorescence Imaging
>>> The Melbourne Materials Institute (MMI)
>>> University of Melbourne, Victoria 3010, Australia
>>> Email: [log in to unmask]<mailto:[log in to unmask]>
>>> Phone: +61 (0)3 9035-7749
>>>
>>>
>>>
>>>
>>>
>>>
>>>
>>> On 20/02/2013, at 7:01 AM, Adam White
>>> <[log in to unmask]<mailto:[log in to unmask]>> wrote:
>>>
>>> *****
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>>> *****
>>>
>>> I did a search of the archive but I think I have exhausted most of the
>>> previous
>>> suggestions... We are attempting to do some overnight live imaging
>>> experiments using 2PE microscopy.  We are using the Nikon Apo LWD 25x/1.1
>>> water immersion objective on an inverted stand and our main problem is
>>> keeping the immersion medium in place for longer than a couple of hours.
>>> We
>>> have tried using the Cargille oil with a 1.335 RI but it does not have
>>> enough
>>> viscosity/surface tension to be useful.  We have tried using ultrasound
>>> gel but
>>> this dries out over time and only gives us about 5 hours of images.  I
>>> have also
>>> tried all manner of different sealants/gloves/o-rings to varying degrees
>>> of
>>> success/reproducibility.  The best I have found is to use a stretched-out
>>> glove
>>> finger (plus a sealant) and just fill up the resulting "reservoir" with
>>> water.  This
>>> has given us enough volume to get about 10 hours worth of imaging but is
>>> hard
>>> to keep consistent.  Obviously, my question is whether someone has
>>> developed
>>> or knows of a better system for doing this?  Perhaps a perfusion system
>>> or a
>>> more durable glove-finger type solution?  I have seen the Leica system
>>> but this
>>> won't fit our objective...  Any input you have to offer would be most
>>> appreciated.
>>>
>>> Best,
>>> Adam
>>>
>>>
>>> Adam B. White, Ph.D.
>>> Confocal & Specialized Microscopy Shared Resource
>>> Herbert Irving Comprehensive Cancer Center
>>> Columbia University
>>> 1130 Saint Nicholas Ave, 222A
>>> New York, NY 10032

-- 
Dan Focht Bioptechs Inc. V: (724)282-7145 www.bioptechs.com