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March 2013

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Confocal Microscopy List <[log in to unmask]>
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Mon, 4 Mar 2013 15:15:59 +1300
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Confocal Microscopy List <[log in to unmask]>
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Dmitry Sokolov <[log in to unmask]>
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*****
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Dear Dave,

thank you for sharing your experience. The review of macroscope 
configurations is being built here:
http://confocal-manawatu.pbworks.com/w/page/16346953/Macroscopes

I have a feeling however that Mark is after something closer to the 
optical configuration close to the stereo microscopes:
http://confocal-manawatu.pbworks.com/w/page/64160949/Field%20of%20View%20of%20Microscopes
That is a matter of the lenses used, of course.

Many thanks again,
Dmitry
*Advanced Knowledge Management*
for *MICROSCOPY *and *Image Analysis *
------------------------------------------------------------------------
*Dmitry Sokolov*, Ph.D.
Mob: *+64 21 063 5382***
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04.03.2013 7:36, Knecht, David ?????:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> I built a macroscope by screwing a zoom/macro lens onto a Sony CCD camera.  That was a number of years ago, but the camera was a XCD-710 (looks like currently Sony’s XCD SX90 at Edmundoptics) and the lens is a Navitar Zoom 7010 lens.  I mounted the camera/lens on a track stand from Howard Electronics.  I use a fiber optic light source like the Dolan Jenner dual fiber optic.  Works great for 1mm to multi-cm sized objects (colonies on petri dishes mostly).  Micro-manger controls image acquisition.  Dave
>
> On Mar 3, 2013, at 12:45 PM, Andrew York wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> Good suggestion! A commercial macro lens might work well. Could you
> recommend a lens, and a monochrome sensor that can stream to a computer?
>
> On Sun, Mar 3, 2013 at 11:20 AM, Mark Cannell <[log in to unmask]<mailto:[log in to unmask]>>wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> 2x2 cm -have you thought about a commercial macro lens?
> Cheers
> On 3/03/2013, at 3:59 PM, Andrew York <
> [log in to unmask]<mailto:[log in to unmask]>> wrote:
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> I'm looking for some advice about buying or building my own low-mag
> brightfield microscope. I want the following capabilities:
>
> * Brightfield illumination
> * Cheap ccd or cmos camera with as many pixels as possible (>10 MP
> ideally), no eyepieces required, acquiring to a computer.
> * Large field of view, ~2x2 cm
> * Adjustable zoom would be nice, over a moderate range (maybe a factor
> of 4)
> * Resolution limited by camera pixel size rather than aberrations or
> diffraction (if possible)
> * Manual controls, no automation required in the optics
> * Room between the objective and the tube lens for a dichroic, which I
> might want to insert later, for free-space coupling of illumination
> beams.
>
> Is there an obvious commercial solution that is good and cheap? If not,
> any
> advice on where to buy the components is appreciated.
>
> Mark  B. Cannell Ph.D. FRSNZ
> Professor of Cardiac Cell Biology
> School of Physiology&  Pharmacology
> Medical Sciences Building
> University of Bristol
> Bristol
> BS8 1TD UK
>
> [log in to unmask]<mailto:[log in to unmask]>
>
>
> David Knecht, Ph.D.
> Professor and Head of Microscopy Facility
> Department of Molecular and Cell Biology
> U-3125
> 91 N. Eagleville Rd.
> University of Connecticut
> Storrs, CT 06269
> 860-486-2200
> 860-486-4331 (fax)

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