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Leica makes a monochrome sensor version of its M body. However, the red 'Leica' badge adds a jaw-dropping price premium. Sigma sells a DSLR with a non-Bayer 'Foveon' sensor that may provide the best alternative; in that case buy a SD15 (~$1k) rather than the overpriced SD1. If not picky then you can get used 10+ MP DSLRs for a song. Whichever way you go interchangeable lenses let you put a very cheap/very good 50mm/1.8 'standard' lens on hollow extension tubes and get a powerful and adjustable macro effect (mag power depends on tube length) for almost no money. Take a look at 'extreme macro' groups on Flickr and elsewhere to see how people do this.
However, things get silly if you need a dichroic. You could machine the hollow extension tubes and jerryrig it (maybe), but at that point you'd spend less time and money on an old inverted scope base and parts from Edmund.
cheers,
TF
Timothy Feinstein, PhD
Visiting Research Associate
Laboratory for GPCR Biology
Dept. of Pharmacology & Chemical Biology
University of Pittsburgh, School of Medicine
BST W1301, 200 Lothrop St.
Pittsburgh, PA 15261
On Mar 3, 2013, at 9:15 PM, Dmitry Sokolov wrote:
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>
> Dear Dave,
>
> thank you for sharing your experience. The review of macroscope configurations is being built here:
> http://confocal-manawatu.pbworks.com/w/page/16346953/Macroscopes
>
> I have a feeling however that Mark is after something closer to the optical configuration close to the stereo microscopes:
> http://confocal-manawatu.pbworks.com/w/page/64160949/Field%20of%20View%20of%20Microscopes
> That is a matter of the lenses used, of course.
>
> Many thanks again,
> Dmitry
> *Advanced Knowledge Management*
> for *MICROSCOPY *and *Image Analysis *
> ------------------------------------------------------------------------
> *Dmitry Sokolov*, Ph.D.
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> 04.03.2013 7:36, Knecht, David ?????:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> I built a macroscope by screwing a zoom/macro lens onto a Sony CCD camera. That was a number of years ago, but the camera was a XCD-710 (looks like currently Sony’s XCD SX90 at Edmundoptics) and the lens is a Navitar Zoom 7010 lens. I mounted the camera/lens on a track stand from Howard Electronics. I use a fiber optic light source like the Dolan Jenner dual fiber optic. Works great for 1mm to multi-cm sized objects (colonies on petri dishes mostly). Micro-manger controls image acquisition. Dave
>>
>> On Mar 3, 2013, at 12:45 PM, Andrew York wrote:
>>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
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>>
>> Good suggestion! A commercial macro lens might work well. Could you
>> recommend a lens, and a monochrome sensor that can stream to a computer?
>>
>> On Sun, Mar 3, 2013 at 11:20 AM, Mark Cannell <[log in to unmask]<mailto:[log in to unmask]>>wrote:
>>
>> *****
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>>
>> 2x2 cm -have you thought about a commercial macro lens?
>> Cheers
>> On 3/03/2013, at 3:59 PM, Andrew York <
>> [log in to unmask]<mailto:[log in to unmask]>> wrote:
>>
>> *****
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>>
>> I'm looking for some advice about buying or building my own low-mag
>> brightfield microscope. I want the following capabilities:
>>
>> * Brightfield illumination
>> * Cheap ccd or cmos camera with as many pixels as possible (>10 MP
>> ideally), no eyepieces required, acquiring to a computer.
>> * Large field of view, ~2x2 cm
>> * Adjustable zoom would be nice, over a moderate range (maybe a factor
>> of 4)
>> * Resolution limited by camera pixel size rather than aberrations or
>> diffraction (if possible)
>> * Manual controls, no automation required in the optics
>> * Room between the objective and the tube lens for a dichroic, which I
>> might want to insert later, for free-space coupling of illumination
>> beams.
>>
>> Is there an obvious commercial solution that is good and cheap? If not,
>> any
>> advice on where to buy the components is appreciated.
>>
>> Mark B. Cannell Ph.D. FRSNZ
>> Professor of Cardiac Cell Biology
>> School of Physiology& Pharmacology
>> Medical Sciences Building
>> University of Bristol
>> Bristol
>> BS8 1TD UK
>>
>> [log in to unmask]<mailto:[log in to unmask]>
>>
>>
>> David Knecht, Ph.D.
>> Professor and Head of Microscopy Facility
>> Department of Molecular and Cell Biology
>> U-3125
>> 91 N. Eagleville Rd.
>> University of Connecticut
>> Storrs, CT 06269
>> 860-486-2200
>> 860-486-4331 (fax)
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