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Hello Pascal,

This is semi commercial response - used to work for Media Cybernetics from
2001-2007, but no longer work for them.

The Image-Pro Plus plugin called AFA is specifically designed to tackle
problems like the one you describe. As Guy suggests what you are looking
for is a contrast based autofocus (not as fast as IR/Laser methods, but
more reliable for anything but thing layers of cells adhered directly to
the coverslip.

Chris Tully
Microscopy and Image Analysis Expert
[log in to unmask]
240-475-9753 (c)

[image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>


On Thu, Mar 7, 2013 at 7:23 AM, Guy Cox <[log in to unmask]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> For your purpose, these IR systems which simply maintain the coverslip at
> a fixed distance from the objective are not what you need, and they were
> never intended for that purpose.  What you need is an image analysis system
> which will search for and find focus (just as a compact digital camera
> will).  I know that SIS (now owned by Olympus) will do this, and I'm sure
> that most other commercial systems will.  There may even be some free
> systems out there.
>
>                                                                Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Pascal Weber
> Sent: Thursday, 7 March 2013 7:33 PM
> To: [log in to unmask]
> Subject: Automatic focus
>
> *****
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>
> Confocal not matter.
>
> I am looking for a microscope that can do focus on slides with bacteria
> without
> any intervention. I would like to use a XY plate with 8 slides, objectiv
> 40X dry.
> Does anyone have an idea? I try IR automatic focus ( Leica, Nikon, Zeiss)
> but
> anyone works correctly.  thank you
>