*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Completely agree - and in addition, unless the bacteria are stained,  
your setup with a 40x dry lens will not give you much useful  
information.  What sort of information DO you want?

On Mar 7, 2013, at 12:33 PM, Julio Vazquez wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> If you have a way to maintain your bacteria in a defined focal plane  
> (agarose gel pad, matrigel, etc...), then IR autofocus should work  
> just fine for you. If the bacteria are not immobilized, then they'll  
> be moving up and down and I am not sure how an image based autofocus  
> will deal with that... it may not be fast enough, and different  
> bacteria will be in different focal planes, so your image based  
> autofocus may get utterly confused...
> --
> Julio Vazquez,
> Fred Hutchinson Cancer Research Center
> Seattle, WA 98109-1024
>
>
> http://www.fhcrc.org/
>
> ==
>
>
> On Mar 7, 2013, at 12:32 AM, Pascal Weber wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Confocal not matter.
>>
>> I am looking for a microscope that can do focus on slides with  
>> bacteria without
>> any intervention. I would like to use a XY plate with 8 slides,  
>> objectiv 40X dry.
>> Does anyone have an idea? I try IR automatic focus ( Leica, Nikon,  
>> Zeiss) but
>> anyone works correctly.  thank you

Robert J. Palmer Jr., Ph.D.
Microbial Receptors Section
Laboratory of Cell and Developmental Biology
Natl Inst Dental Craniofacial Res - Natl Insts Health
Bldg 30, Room 207
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396