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Dear Michal,

manufacturers state the standard use which is simply what the antibody 
is tested for (and they guarantee that it works). Especially, it is 
important to know whether the antibody reacts against the native or the 
denatured form of the protein. The latter would be for WB analysis, the 
previous for IF. That however, does not exclude that the very same 
antibody might also work in the other application. I have had a few 
antibodies working in both, some however did not.

As for all images, just do the proper controls, the ones you should also 
employ when using an specifically developped antibody, e.g. incubate 
with the secondary alone, titrate the antibody, check against your 
biological negative control (if you have none, try to come up with one 
that makes sense); if you are looking for special subcellular 
compartments try to confirm with a second staining (e.g. the ER has 
different domains labeled differentially with various antibodies all 
sold as if they would recognize "the" ER...) and so on.

So as for your original question: No, I would not do this with other 
IgGs - depending on your setup and question I would rather employ other 
controls.

Johannes


*Dr. Johannes Koch*

*Tissue Med Biosciences GmbH*

Magnesitstrasse1 | A‑3500 Krems

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Am 08.03.2013 15:27, schrieb Tineke Vendrig:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
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>
> It depends on the kind of antibodies. How are these made? In mice ot rats.
> or....What is the titer? You can try it anywhere..??
> Best,
> Tineke Vendrig
>
> 2013/3/8 Michał Majkowski <[log in to unmask]>
>
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>>
>> Dear All,
>> I do not know if this is a good place to address my problem as it regards
>> immunofluorescence. The problem is: we have in the lab antibodies dedicated
>> to Western Blot (according to manufacturer; Abcam). We used them to IF and
>> we obtained some nice images. What do you think about such data? Should it
>> be confirmed by IgG dedicated to IF?   Thank you for help.
>> Best,
>> Michal
>>