CONFOCALMICROSCOPY Archives

March 2013

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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Richard Cole <[log in to unmask]>
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Date:
Wed, 13 Mar 2013 09:41:05 -0400
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Dear All,

 

Immersion lens, especially high NA ones:  Although not simple to setup, the
most accurate way to measure transmittance/power is to use two objectives
focused on a coverslip sandwich.   The position in XY&Z can be tweaked to
give the maximum power.  Infinity corrected lens this is easier.  I would be
happy to provide more details off line.

 

Happy imaging

 

Rich

 

Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center

 

Research Assistant Professor 
Dept. of Biomedical Sciences 
School of Public Health State University of New York 


P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax 

 

Email  <mailto:[log in to unmask]> [log in to unmask]

Website  <http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm

 

 

 

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