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Dear All,
Immersion lens, especially high NA ones: Although not simple to setup, the
most accurate way to measure transmittance/power is to use two objectives
focused on a coverslip sandwich. The position in XY&Z can be tweaked to
give the maximum power. Infinity corrected lens this is easier. I would be
happy to provide more details off line.
Happy imaging
Rich
Richard Cole
Research Scientist V
Director: Advanced Light Microscopy Core Unit
Wadsworth Center
Research Assistant Professor
Dept. of Biomedical Sciences
School of Public Health State University of New York
P.O. Box 509 Albany N.Y. 12201-0509
518-474-7048 Phone
518-474-4430 Fax
Email <mailto:[log in to unmask]> [log in to unmask]
Website <http://www.wadsworth.org/cores/alm/index.htm>
www.wadsworth.org/cores/alm/index.htm