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Richard, that sounds like an interesting protocol. I'd like to learn about it.

John Oreopoulos

On 2013-03-13, at 9:41 AM, Richard Cole <[log in to unmask]> wrote:

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> 
> Dear All,
> 
> 
> 
> Immersion lens, especially high NA ones:  Although not simple to setup, the
> most accurate way to measure transmittance/power is to use two objectives
> focused on a coverslip sandwich.   The position in XY&Z can be tweaked to
> give the maximum power.  Infinity corrected lens this is easier.  I would be
> happy to provide more details off line.
> 
> 
> 
> Happy imaging
> 
> 
> 
> Rich
> 
> 
> 
> Richard Cole
> Research Scientist V
> Director: Advanced Light Microscopy Core Unit
> Wadsworth Center
> 
> 
> 
> Research Assistant Professor 
> Dept. of Biomedical Sciences 
> School of Public Health State University of New York 
> 
> 
> P.O. Box 509 Albany N.Y. 12201-0509
> 518-474-7048 Phone
> 518-474-4430 Fax 
> 
> 
> 
> Email  <mailto:[log in to unmask]> [log in to unmask]
> 
> Website  <http://www.wadsworth.org/cores/alm/index.htm>
> www.wadsworth.org/cores/alm/index.htm
> 
> 
> 
> 
> 
>