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Thank you for the many replies.

Our sales rep just stopped in and the email below explains the problem.

People in the lab were using the #1 coverslip bottom wells and because of an issue on the TIRF microscope and my formal microscopy training, I insisted we switch to 1.5 coverslips.  We did not realize that the entire design was different.

According to the sales rep, both the old design as #1 and the new design as #1.5 are available.  So I'm going to recommend that for normal confocal etc. people use the #1 chambers even though the optics aren't perfect.  And we will try snipping off or otherwise removing the tabs on the newer models.

Thanks again!

Regards,

Michael




-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of els vanstreels
Sent: Wednesday, March 13, 2013 11:54 AM
To: [log in to unmask]
Subject: Re: Nunc Lab-Tek problem

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 Hi Michael,
The difference is between the Labtek and the Labtek II version of the chambered coverglass. The first one has the no. 1 and the second has the no
1.5 coverglass bottom.  As far as I know both models are still available (at least over here..).  Whenever we us the II version, we simply cut off the tab with an ordinary wire-shear.

Best regards

Els




-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Cammer, Michael
Sent: woensdag 13 maart 2013 16:17
To: [log in to unmask]
Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem

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Hi.

For many years we used Nunc Lab-Tek chambered coverglasses. They were great.

However, they recently began shipping a different design with the same product number. The chambers no longer fit in our microscope stages inserts.
Also, the volume changed.
see http://www.flickr.com/photos/mcammer/sets/72157632984171809/

While we can adjust to the volume change, the plastic tab at the end of the chamber is a big problem.

I thought of having people cut the tab off before doing experiments, but this would produce a lot of plastic dust (and we'd have to buy a suitable saw or dremel).

Does anybody know of an alternative manufacturer or if the old style chambers are still available from somewhere?

Thanks!

Michael




________________________________________________________
Michael Cammer, Assistant Research Scientist Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208  Cell: (914) 309-3270