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We have two Pathology Devices stage top incubators (http://www.pathologydevices.com).  Dishes and chambers sit into the device so there is little loss of atmosphere around the objective.  They read out the gas concentration inside the chamber and feedback regulate.  The humidity is from a heated water holder.  When we tested a few years ago, it was the only device we tested that actually maintained volume over many hours to overnight incubation.  Dave

On Mar 13, 2013, at 1:32 PM, B. Prabhakar Pandian wrote:

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Hello All,
                 We are looking to buy a stage top incubator. However, one of the questions which has come up is whether the gases are running continuously to maintain levels since it is open near the objective.
Are there gaskets to seal around slides/petri dishes so that a feedback on the levels can be provided and the gas flow can be upregulated or downregulated depending on the need.   Finally, most of them
have a bottle of water for humidifying the chamber. Only Tokai Hit has tray with water in the insert itself. Any thoughts on the pros/cons is appreciated.

Any other feedback to help us make a sound decision will be great.

Thanks,

-Prabhakar

David Knecht, Ph.D.
Professor and Head of Microscopy Facility
Department of Molecular and Cell Biology
U-3125
91 N. Eagleville Rd.
University of Connecticut
Storrs, CT 06269
860-486-2200
860-486-4331 (fax)