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I use these #1.5 Labtek II slides routinely for intracellular FCS 
measurements, without removing the tab. The trick is that you place 
these chambers in the perpendicular direction in your standard holder 
and not in the usual one.

Standard: long side from left to right, if you look over the eyepieces 
on your stage
Labtek II: short side from left to right and long side from the 
eyepieces to the brightfield arm

At least in our standard holders from Zeiss and Leica they fit quiet 
well in that way, the only problem is that you can not use the whole 
area of the outer chambers, because (especially with High NA Zeiss 
objectives) the objective will touch the stage holder.

Bye Wolfgang


Am 13.03.2013 20:11, schrieb Cammer, Michael:
>
> Thank you for the many replies.
>
> Our sales rep just stopped in and the email below explains the problem.
>
> People in the lab were using the #1 coverslip bottom wells and because 
> of an issue on the TIRF microscope and my formal microscopy training, 
> I insisted we switch to 1.5 coverslips.  We did not realize that the 
> entire design was different.
>
> According to the sales rep, both the old design as #1 and the new 
> design as #1.5 are available.  So I'm going to recommend that for 
> normal confocal etc. people use the #1 chambers even though the optics 
> aren't perfect.  And we will try snipping off or otherwise removing 
> the tabs on the newer models.
>
> Thanks again!
>
> Regards,
>
> Michael
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List 
> [mailto:[log in to unmask]] On Behalf Of els vanstreels
> Sent: Wednesday, March 13, 2013 11:54 AM
> To: [log in to unmask]
> Subject: Re: Nunc Lab-Tek problem
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=nfocalmicroscopy
> *****
>
>  Hi Michael,
> The difference is between the Labtek and the Labtek II version of the 
> chambered coverglass. The first one has the no. 1 and the second has 
> the no
> 1.5 coverglass bottom.  As far as I know both models are still 
> available (at least over here..).  Whenever we us the II version, we 
> simply cut off the tab with an ordinary wire-shear.
>
> Best regards
>
> Els
>
>
>
>
> -----Original Message-----
> From: Confocal Microscopy List 
> [mailto:[log in to unmask]] On Behalf Of Cammer, Michael
> Sent: woensdag 13 maart 2013 16:17
> To: [log in to unmask]
> Subject: [CONFOCALMICROSCOPY] Nunc Lab-Tek problem
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=nfocalmicroscopy
> *****
>
> Hi.
>
> For many years we used Nunc Lab-Tek chambered coverglasses. They were 
> great.
>
> However, they recently began shipping a different design with the same 
> product number. The chambers no longer fit in our microscope stages 
> inserts.
> Also, the volume changed.
> see http://www.flickr.com/photos/mcammer/sets/72157632984171809/
>
> While we can adjust to the volume change, the plastic tab at the end 
> of the chamber is a big problem.
>
> I thought of having people cut the tab off before doing experiments, 
> but this would produce a lot of plastic dust (and we'd have to buy a 
> suitable saw or dremel).
>
> Does anybody know of an alternative manufacturer or if the old style 
> chambers are still available from somewhere?
>
> Thanks!
>
> Michael
>
>
>
>
> ________________________________________________________
> Michael Cammer, Assistant Research Scientist Skirball Institute of 
> Biomolecular Medicine
> Lab: (212) 263-3208  Cell: (914) 309-3270
> </pre></body>
> </html>
> </html>

-- 
Dr. Wolfgang Staroske

Single Molecule Specialist
Light Microscopy Facility

Technische Universität Dresden
Biotechnology Center
Tatzberg 47/49
01307 Dresden, Germany

Tel.: +49 (0) 351 463-40316
Fax.: +49 (0) 351 463-40342
E-Mail: [log in to unmask]
Webpage: www.biotec.tu-dresden.de