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Unfortunately a tinted incubator was not ordered as part of this rig and it
is a rather complicated enclosure (lots of ins & outs) and took 3+ fittings
to get it to fit right, so while it would be relatively easy to replicate
now, I would rather not pay the additional expense (and have a useless
clear enclosure)... but that might be the best option.

The primary wavelengths that needs to be blocked are 561nm and 450nm (PALM
scope), but there are also several other wavelength lasers (458, 488, 514,
633nm), although the power is much less on those.

I will check out this theatrical filter - thanks for the idea, Guy!

(and yes, we have laser safety goggles for alignments)

Thanks,

-Holly

(transitioning to bMail...  thank you for your patience)

___________________________
Holly L. Aaron
Molecular Imaging Center
Cancer Research Laboratory
University of California Berkeley
251 LSA #2751
Berkeley, CA  94720-2751
510.642.2901
510.642.5741 fax
[log in to unmask]
http://imaging.berkeley.edu

*
*





On Tue, Mar 19, 2013 at 11:02 AM, Gregg Jarvis <[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> What WL is the Laser?
> You should probably be wearing Laser Safety Goggles.
> If you are just trying to minimize scatter so you don't look at it then
> window tint might work.
> Do they make a tinted incubator that you can buy that is for your scope?
> The local Autozone will have different shades of darkness to choose from,
> $14
>
> On Tue, Mar 19, 2013 at 12:30 PM, Holly L. AARON <[log in to unmask]
> >wrote:
>
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
> >
> > Hi, All - I apologize for the non-confocal nature of this question, but I
> > cannot think of a better resource than this community. I am looking for
> > some sort of "tinting" or similar to minimize the laser exposure through
> a
> > clear plastic incubation chamber on a TIRF-based rig.  Our laser safety
> > officer suggested tinting would be okay, but I have no idea where to look
> > for that in custom sizes/amounts. Would window tinting for vehicles work?
> > Has anyone done this? Would love to hear your experience.
> >
> > Thanks as always!
> > -Holly
> > ___________________________
> > Holly L. Aaron
> > Molecular Imaging Center
> > Cancer Research Laboratory
> > University of California Berkeley
> > 251 LSA #2751
> > Berkeley, CA  94720-2751
> > 510.642.2901
> > 510.642.5741 fax
> > [log in to unmask]
> > http://imaging.berkeley.edu
> >
> > *
> > *
> >
>
>
>
> --
> Gregg Jarvis
> Senior Spectroscopist
> Advanced Products Group
> Omega Optical Inc.
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