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You could use reflection mode from the surface of the slide to see if it's moving?

Cheers

On 21/03/2013, at 10:40 AM, Christof Schwiening <[log in to unmask]> wrote:

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> Dear Glen,
> The solutions are gravity-fed via independent tubes from the base of 50 ml 
> syringes suspended about 50cm above the microscope stage. The tubes are 
> identical and are connected to electrical on-off valves (General Valves). The 
> tubes meet at a manifold about 15 cm from the output. In the case of the 
> video the output is directly onto the top of a fixed-preparation (Convallaria) 
> slide which was mounted directly onto the fixed platform of the microscope. 
> The output tube was about 2 mm from the objective at the level of the slide. 
> The suction was similarly sighted about 2 mm from the objective at an angle of 
> 180 degrees to the input. The suction was from a bottle (2 L) maintained a 
> low pressure with a high frequency low volume pump. The only oscillations that 
> occur within the system are surface tension effects on the suction when air 
> mixes with the water in the output lines - this occurs all of the time and 
> sounds like a child sucking through a straw from an empty glass of lemonade!
> I doubt that it is movement. I guess I would need a force transducer mounted 
> on the slide to prove that.
> Greetings,
> Christof

Mark  B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology&  Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK

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