CONFOCALMICROSCOPY Archives

March 2013

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Subject:
Re: Automatic focus
From:
Johan Henriksson <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Thu, 7 Mar 2013 15:16:31 +0100
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*****
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*****

there is supposedly a software autofocus in micro-manager (
www.micro-manager.org), but I haven't had reason to use it myself yet

these algorithms, and there is more than one, will try to find the plane
with the best contrast (=best focus(?)) but if the one above is not good
enough, you can add you own algorithm

/Johan

On Thu, Mar 7, 2013 at 1:23 PM, Guy Cox <[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> For your purpose, these IR systems which simply maintain the coverslip at
> a fixed distance from the objective are not what you need, and they were
> never intended for that purpose.  What you need is an image analysis system
> which will search for and find focus (just as a compact digital camera
> will).  I know that SIS (now owned by Olympus) will do this, and I'm sure
> that most other commercial systems will.  There may even be some free
> systems out there.
>
>                                                                Guy
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of Pascal Weber
> Sent: Thursday, 7 March 2013 7:33 PM
> To: [log in to unmask]
> Subject: Automatic focus
>
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Confocal not matter.
>
> I am looking for a microscope that can do focus on slides with bacteria
> without
> any intervention. I would like to use a XY plate with 8 slides, objectiv
> 40X dry.
> Does anyone have an idea? I try IR automatic focus ( Leica, Nikon, Zeiss)
> but
> anyone works correctly.  thank you
>



-- 
-- 
-----------------------------------------------------------
Johan Henriksson, PhD
Karolinska Institutet
Ecobima AB - Custom solutions for life sciences
http://www.ecobima.com  http://mahogny.areta.org  http://www.endrov.net

<http://www.endrov.net>

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