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If you have a way to maintain your bacteria in a defined focal plane (agarose gel pad, matrigel, etc...), then IR autofocus should work just fine for you. If the bacteria are not immobilized, then they'll be moving up and down and I am not sure how an image based autofocus will deal with that... it may not be fast enough, and different bacteria will be in different focal planes, so your image based autofocus may get utterly confused...
--
Julio Vazquez,
Fred Hutchinson Cancer Research Center
Seattle, WA 98109-1024


http://www.fhcrc.org/

==


On Mar 7, 2013, at 12:32 AM, Pascal Weber wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Confocal not matter.
> 
> I am looking for a microscope that can do focus on slides with bacteria without 
> any intervention. I would like to use a XY plate with 8 slides, objectiv 40X dry. 
> Does anyone have an idea? I try IR automatic focus ( Leica, Nikon, Zeiss) but 
> anyone works correctly.  thank you