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If speed is the issue and you can illuminate with broad spectrum light, you
might want to consider the QV2 from Photometrics:
http://www.photometrics.com/products/multichannel/

It allows a single camera to simultaneously image up to 4 channels by
splitting the chip into quadrants and splitting the light into four paths
that each pass through a different emission filter before hitting the CCD.

Just clarify, I have never personally used one of these in a research
project, but I have worked with one in developing software support for the
resulting images. I do not work for Photometrics.

Chris Tully
Microscopy and Image Analysis Expert
[log in to unmask]
240-475-9753 (c)

[image: View my profile on LinkedIn]<http://www.linkedin.com/in/christully/>


On Tue, Mar 5, 2013 at 2:09 PM, Jean-Pierre CLAMME <
[log in to unmask]> wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hi Andreas,
>
> The issue is not grouping channels in the sequential box and choosing line
> and frame. The issue is changing filter sets quickly. To take the 3 images
> DD, DA, AA the filter/light pathway has to be changed between images. DD
> and DA  or DD and AA can be taken in the same configuration, but AA and DA
> can't. So the only way I can see is to use the Virtual channels and that
> is too slow line by Line.
>
> Best
>
> JP
>
>
>
>
>
> Confocal Microscopy List <[log in to unmask]> wrote on
> 03/05/2013 07:01:06 AM:
>
> > Andreas Bruckbauer <[log in to unmask]>
> > Sent by: Confocal Microscopy List <[log in to unmask]>
> >
> > 03/05/2013 07:05 AM
> >
> > Please respond to
> > Confocal Microscopy List <[log in to unmask]>
> >
> > To
> >
> > [log in to unmask]
> >
> > cc
> >
> > Subject
> >
> > Re: Ratiometric FRET on Fluoview
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
>
> > I have not used it for FRET, but we have a sequential tick box at
> > the bottom of the window with the channel parameters. When ticked we
> > can select "frame by frame" or "line by line" aquisition and how the
> > channels will be grouped together.
>
> > best wishes
>
> > Andreas
>
> >
> > -----Original Message-----
> > From: Jean-Pierre CLAMME <[log in to unmask]>
> > To: CONFOCALMICROSCOPY <[log in to unmask]>
> > Sent: Mon, 4 Mar 2013 22:44
> > Subject: Ratiometric FRET on Fluoview
>
> >
> > *****
> > To join, leave or search the confocal microscopy listserv, go to:
> > http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> > *****
>
> > Hello,
>
> > I saw a paper about Ratiometric FRET (Roszik, Cytometer 2009) mentioning
> > line by line acquisition of the 3 images (IDA, IDD, IAA). The authors
> > mentioned the use of a LSM 510.
>
> > I don't know the LSM510, but I have a fluoview 1000 and the only way I
> can
> > see how to do that is using the "virual Channels" . However that would
> be
> > image by image and not line by line.
>
> > Does anyone has done ratiometric FRET on the fluoview and what method
> did
> > you use ?
>
> > Thank you and Best regards,
>
> > JP
>
> >
> > - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
> > Jean-Pierre CLAMME, PhD
> > Chief Scientist
> > Nitto Denko Technical
> > 501 Via Del Monte
> > Oceanside, CA 92058
> > E-mail: [log in to unmask]
> > Phone: 1-760-696-9428
>