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What Mark said happened to a Leica objective on our inverted microscope. That objective was stuck on the turret. I saw some light green stuff deposited around the circumference of the objective rear thread. After calling Leica rep, I clean the green stuff with dipping DI water to Q-tips to dissolve the deposit carefully. The light green deposit was most likely copper chlorite, as one group frequently imaged live cell in Tyrode's solution, which contains several chlorites. One user could dropped the solution by accident.
I would try warm DI water first, as some suggested already.
Best regards,
Jingsong Wang, PhD
Imaging Core Facilty
Child & Family Research Institute
Room A4-110, 950 W. 28th. Ave.
Vancouver BC V5Z 4H4 Canada
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Mark Cannell
Sent: April-29-13 11:00 PM
To: [log in to unmask]
Subject: Re: Advice on lens cleaning
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I don't know if anyone said this, but the crystals could be an inorganic component? The green colour could be from the salt leaching copper from the brass of the objective. I would try hot (not boiling) water plus detergent first before moving up the scale of aggressive solvents .(Speaking from experience having re-glued an objective lens due to overzealous use of acetone by a colleague -a tricky operation down a stereo microscope).
HTH
Mark
On 29/04/2013, at 11:10 PM, Craig Brideau <[log in to unmask]> wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> What John said, but maybe try a Methanol soak before resorting to toluene.
> I find it does a decent job of breaking up lens oil in general. I'm
> not sure how it will work on your crystals, but if the warm water bath
> doesn't work, maybe try a methanol bath?
>
> Craig
>
>
> On Mon, Apr 29, 2013 at 12:43 PM, John Oreopoulos <
> [log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Gabriel,
>>
>> I should stress that my toluene soaking suggestion has never been
>> tried here in house and was only suggested as a last ditch effort to
>> save a lens in a dire situation like the one you described. The other
>> suggestions made already seem much "milder" and should probably be
>> tried before the method I mentioned. Try at your own discretion!...
>> And do let us know how it all goes afterwards.
>>
>> Cheers,
>>
>> John Oreopoulos
>>
>>
>> On 2013-04-27, at 7:21 AM, phil laissue wrote:
>>
>>> *****
>>> To join, leave or search the confocal microscopy listserv, go to:
>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>> *****
>>>
>>> The tiniest bit of (warm) distilled water in a small beaker, just
>>> enough to touch the front lens, may do the trick. And Zeiss also had
>>> a crystallising immersion oil several years ago, without green
>>> crystals though.
>>> _____________________________________
>>> Philippe Laissue, PhD, Bioimaging Manager School of Biological
>>> Sciences, Room 4.17 University of Essex, Colchester CO4 3SQ, UK
>>> (0044) 01206 872246 / (0044) 07842 676 456 [log in to unmask]
>>> privatewww.essex.ac.uk/~plaissue
>>>
>>>
>>> On Sat, Apr 27, 2013 at 11:23 AM, Matthew Nicholas
>>> <[log in to unmask]> wrote:
>>>> *****
>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>> *****
>>>>
>>>> I've never dealt with this problem before, so this may be off the
>>>> mark,
>> but
>>>> what about using more of the oil itself to dissolve the crystals?
>>>>
>>>> Good luck!
>>>> Matt
>>>>
>>>> On 4/26/13 10:47 PM, Tina Carvalho wrote:
>>>>>
>>>>> *****
>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>> *****
>>>>>
>>>>> True, that. I had green crystals form in Leica oil as well! I
>>>>> can't remember for sure, but I think we heated it to dissolve
>>>>> them, or at
>> least
>>>>> mobilize the oil enough that could be gently wiped off before
>>>>> trying
>> any
>>>>> cleaner.
>>>>>
>>>>> Good luck and aloha,
>>>>> Tina
>>>>>
>>>>>>
>>>>>> I should add that the oil with the crystals was from Leica.
>>>>>> Joel
>>>>>>
>>>>>>
>>>>>> On Fri, Apr 26, 2013 at 8:30 PM, JOEL B. SHEFFIELD
>>>>>> <[log in to unmask]>
>>>>>> wrote:
>>>>>>
>>>>>>> Wow! Toluene? We have had the experience of immersion oil
>> crystalizing
>>>>>>> in the vial. Heating it slightly made the crystals dissolve.
>> Perhaps a
>>>>>>> warm bath would be helpful in this case, or just warming the
>>>>>>> lens to
>> 38
>>>>>>> or
>>>>>>> so..
>>>>>>>
>>>>>>> Joel
>>>>>>>
>>>>>>> On Fri, Apr 26, 2013 at 6:57 PM, John Oreopoulos <
>>>>>>> [log in to unmask]> wrote:
>>>>>>>
>>>>>>>> *****
>>>>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>>>>> *****
>>>>>>>>
>>>>>>>>> Hey Gabriel,
>>>>>>>>>
>>>>>>>>> I consulted with one of the optics experts over here and he
>> recommends
>>>>>>>>
>>>>>>>> the following:
>>>>>>>>>
>>>>>>>>>
>>>>>>>>> Fill a beaker with toluene and create a rig above the beaker
>>>>>>>>> that
>> will
>>>>>>>>
>>>>>>>> allow you to hold the tip of the objective with the oily
>>>>>>>> crystals submerged in the toluene. Leave it there for a week,
>>>>>>>> take it out and then re-examine the lens. Hopefully you can use
>>>>>>>> standard cleaning procedures after
>> that
>>>>>>>> for
>>>>>>>> any residual muck. Best of luck!
>>>>>>>>>
>>>>>>>>>
>>>>>>>>> John Oreopoulos
>>>>>>>>> Staff Scientist
>>>>>>>>> Spectral Applied Research
>>>>>>>>> Richmond Hill, Ontario
>>>>>>>>> Canada
>>>>>>>>> www.spectral.ca
>>>>>>>>>
>>>>>>>>> On 2013-04-26, at 2:03 PM, Gabriel Lapointe <
>>>>>>>>
>>>>>>>> [log in to unmask]> wrote:
>>>>>>>>>
>>>>>>>>>
>>>>>>>>>> *****
>>>>>>>>>> To join, leave or search the confocal microscopy listserv, go to:
>>>>>>>>>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>>>>>>>>>> *****
>>>>>>>>>>
>>>>>>>>>> I have a problem with a dirty objective installed on a
>>>>>>>>>> straight
>> Leica
>>>>>>>>>> microscope. The lens is covered in what looks like oil mixed
>>>>>>>>>> with solid dark green particles and the metal edge with green
>>>>>>>>>> oily crystal.
>> That
>>>>>>>>>> system hasn't been used for a while so I'm inclined to think
>>>>>>>>>> that
>>>>>>>>
>>>>>>>> immersion
>>>>>>>>>>
>>>>>>>>>> oil was left on the objective for more than a year.
>>>>>>>>>>
>>>>>>>>>> Have you ever seen anything like that and do you have any
>> suggestion
>>>>>>>>
>>>>>>>> as to
>>>>>>>>>>
>>>>>>>>>> how I should start the cleaning? I'm afraid to use my
>>>>>>>>>> standard cleaning protocol as the particles inside the oil
>>>>>>>>>> could scratch the lens.
>>>>>>>>>>
>>>>>>>>>> Thanks for your help.
>>>>>>>>>> *Gabriel Lapointe, M.Sc.*
>>>>>>>>>> Lab Manager / Microscopy Specialist Concordia University,
>>>>>>>>>> Biology Department
>>>>>>>>>> 7141 Sherbrooke St. West SP 534 Montréal QC H4B 1R6 Canada
>>>>>>>>>> Lab : (514) 848-2424 x5988 Office : (514) 848-2424 x3008 Fax
>>>>>>>>>> : (514) 848-2881 Cell : (514) 278-0247
>>>>>>>>>> [log in to unmask] cmac.concordia.ca
>>>>>>>>>> http://gabriellapointe.ca
>>>>>>>>
>>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>>
>>>>>>> --
>>>>>>>
>>>>>>>
>>>>>>> Joel B. Sheffield, Ph.D
>>>>>>> Department of Biology
>>>>>>> Temple University
>>>>>>> Philadelphia, PA 19122
>>>>>>> Voice: 215 204 8839
>>>>>>> e-mail: [log in to unmask]
>>>>>>> URL: http://astro.temple.edu/~jbs
>>>>>>>
>>>>>>
>>>>>>
>>>>>>
>>>>>> --
>>>>>>
>>>>>>
>>>>>> Joel B. Sheffield, Ph.D
>>>>>> Department of Biology
>>>>>> Temple University
>>>>>> Philadelphia, PA 19122
>>>>>> Voice: 215 204 8839
>>>>>> e-mail: [log in to unmask]
>>>>>> URL: http://astro.temple.edu/~jbs
>>>>>>
>>>>>
>>>>>
>>>>>
>> *********************************************************************
>> *******
>>>>> * Tina (Weatherby) Carvalho * [log in to unmask]
>>>>> *
>>>>> * Biological Electron Microscope Facility * (808) 956-6251
>>>>> *
>>>>> * University of Hawaii at Manoa *
>>>>> http://www.pbrc.hawaii.edu/bemf*
>>>>>
>>>>>
>> *********************************************************************
>> *******
>>>>
>>>>
>>>> --
>>>> Matthew Nicholas
>>>> Medical Scientist Training Program Student Laboratory of Arne
>>>> Gennerich Department of Anatomy and Structural Biology Albert
>>>> Einstein College of Medicine Forchheimer Building, Room 628
>>>> 1300 Morris Park Avenue
>>>> Bronx, New York 10461
>>>> 718.430.3446
>>>> [log in to unmask]
>>
Mark B. Cannell Ph.D. FRSNZ
Professor of Cardiac Cell Biology
School of Physiology & Pharmacology
Medical Sciences Building
University of Bristol
Bristol
BS8 1TD UK
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