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Hi,

I'm using MEA/cysteamine as a reducing agent in a STORM (super resolution)
buffer. As I understand, it is quite sensitive to water and humidity. I
have stored the powder bottle at -20°C when I received it, then brought it
back to RT, aliquoted it in small amount of powder (60-100 mg) in 1.5 mL
Eppedorf as stated in the protocol I'm following. However this protocol
asks to dessicate the powder aliquots before putting them back to -20°C. As
I didn't know how to proceed, I stored them back to -20°C without
dessicating them.
So a question for the chemists here: is the dessication part really useful
if the product is stored in a freezer? If so, is it a good idea to
dessicate them now, ie to have them do another -20°C / RT /-20°C cycle in
order to dry them?

Thanks for your advices,

Christophe

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Christophe Leterrier
Researcher
Axonal Domains Architecture Team
CRN2M CNRS UMR 7286
Aix Marseille University, France