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To which I would add that each of the antibodies must be labeled directly
--no secondary ab. Nevertheless, quite a tour de force.

Joel


On Wed, Jul 3, 2013 at 12:18 PM, Kilgore, Jason
<[log in to unmask]>wrote:

> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Interesting.  61 different targets on the same paraffin section.
>
> To be somewhat pedantic, when I think of "plex"-ing I think of concurrent
> labeling.  Really, as I understand it, this example is sequential labeling
> and imaging, with a patented "fluorophore inactivation" solution in between
> each labeling and imaging cycle, with a cy dye.  You still have to deal
> with autofluorescence and taking the exact same view for each imaging
> session to overlay.
>
> But a very novel method.
>
> Jason
>
>
> -----Original Message-----
> From: Confocal Microscopy List [mailto:[log in to unmask]]
> On Behalf Of George McNamara
> Sent: Tuesday, July 02, 2013 6:14 PM
> To: [log in to unmask]
> Subject: 60plex immunofluorescence microscopy
>
> *****
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>
> Dear Confocal listserv members,
>
> No need to have plex envy when your flow cytometry colleagues talk
> about  ~20plex in fluorescence flow cytometry or ~40plex in CyTOF mass
> cytometry (or 10plex on the EMD Millipore/AMNIS ImageStreamX imaging in
> flow cytometer). Here are the links to recent papers on "60plex"
> immunofluorescence microscopy (and should be able to do more).
>
>
> new Gerdes et al GE  multiplex paper in PNAS (open access)
>
> http://www.pnas.org/content/early/2013/06/27/1300136110.full.pdf
>
> Gerdes PNAS supplemental files are at
>
> http://www.pnas.org/content/early/2013/06/27/1300136110/suppl/DCSupplemental
>
>
> And another Gerdes (Nelson) et al multiplex paper (also open access)
> http://bio.biologists.org/content/2/5/439.abstract
>
> In addition to GE, see
> http://www.chipcytometry.com/technology.phtml
>
>
> Enjoy,
>
> George
>



-- 


Joel B. Sheffield, Ph.D
Department of Biology
Temple University
Philadelphia, PA 19122
Voice: 215 204 8839
e-mail: [log in to unmask]
URL:  http://astro.temple.edu/~jbs