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Have you checked out the Invitrogen spectra viewer?
http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Labeling-Chemistry/Fluorescence-SpectraViewer.html

According to that you should be ok, but you will only be catching the tail
of the dye with any practical filter set so you will suffer significant
signal loss. You might still have enough to work with depending on
concentration, laser power, etc.

Craig
On 2013-07-03 12:38 PM, "Scott Wong" <[log in to unmask]> wrote:

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>
> Hi everyone,
> I was wondering if anyone has successfully used the Alexa Fluor 532
> secondary to view tyrosine hydroxylase in dopaminergic neurons. I'll be
> imaging it using a 543nm laser with a Nikon confocal microscope. Based on
> its
> excitation wavelength it seems to be an ideal match but I don't any
> experience with it.
>
> If not, can anyone comment on its anti-quenching properties? I'm currently
> using the Alexa Fluor 546 for the same purpose but it quenches almost
> unusably fast with every mounting media I've tried.
>
> Thanks for the help,
> Scott Wong
>