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Hi Craig,
Thanks for the link. The Alexa Fluor 532 and 555 both seem better suited
for the laser I'm using according the the spectraviewer. A few people have
commented that the 532 also quenches quite fast though so I'll most likely
end up getting the 555.
Cheers,
Scott
>
> Have you checked out the Invitrogen spectra viewer?
> http://www.invitrogen.com/site/us/en/home/Products-and-Services/Applications/Cell-Analysis/Labeling-Chemistry/Fluorescence-SpectraViewer.html
>
> According to that you should be ok, but you will only be catching the tail
> of the dye with any practical filter set so you will suffer significant
> signal loss. You might still have enough to work with depending on
> concentration, laser power, etc.
>
> Craig
> On 2013-07-03 12:38 PM, "Scott Wong" <[log in to unmask]> wrote:
>
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Hi everyone,
>> I was wondering if anyone has successfully used the Alexa Fluor 532
>> secondary to view tyrosine hydroxylase in dopaminergic neurons. I'll be
>> imaging it using a 543nm laser with a Nikon confocal microscope. Based
>> on
>> its
>> excitation wavelength it seems to be an ideal match but I don't any
>> experience with it.
>>
>> If not, can anyone comment on its anti-quenching properties? I'm
>> currently
>> using the Alexa Fluor 546 for the same purpose but it quenches almost
>> unusably fast with every mounting media I've tried.
>>
>> Thanks for the help,
>> Scott Wong
>>
>