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July 2013

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Subject:
Neo scMOS problem
From:
Lu Lei <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Jul 2013 02:56:54 -0500
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Hi all,
We recently purchased a wide-field microscope system with a scMOS camera 
(Neo, 5.5 Megapixel, by Andor). We are having quite some annoying problems 
with this Neo scMOS camera. Though it is a 16-bit device, it does not have the 
dynamic range it should have in our system.
1)	In “Rolling” shutter mode, the intensity value that the camera could 
generate is truncated at ~20K. In another word, the camera saturates at ~20K 
instead of 65535.
2)	In “Global” shutter mode, the camera saturates at ~57K—a little short of 
65535.
The camera is controled by the latest MetaMorph. The cooling temperature of 
the camera seems also normal as displayed by MetaMorph (-30&#61616;C). We haven’t 
received any explanation from Andor via local vendor yet. I am wondering what 
is configured incorrectly here. 
Does anyone have similar experience of this camera or suggestion to share?
Thanks a lot.
Regards,
Lei Lu
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Lu Lei (Asst Prof) | School of Biological Sciences | Nanyang Technological 
University SBS-03n-18, 60 Nanyang Drive, Singapore 637551
Tel: (65) 6592-2591 | Fax: (65) 6791-3856 | Email: [log in to unmask]
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