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July 2013

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Subject:
Re: Neo scMOS problem
From:
Orla Hanrahan <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 26 Jul 2013 16:43:59 +0000
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Hi Lei,

Your Neo sCMOS camera has a dynamic range of 30,000:1 (e-s).  The dynamic range of a camera is typically defined as the full-well capacity divided by the camera noise and relates to the ability of a camera to record simultaneously very low light signals alongside bright signals.  From the dynamic range of the Neo, this means that a pixel can hold a maximum signal of 30,000 photoelectrons and will only have 1 electron noise.  

In order to convert these photoelectrons into counts the sensitivity and the read out speed of the camera have to be taken into account. The sensitivity of the Neo is dependent on the amplifier used (i.e. 11-bit or 16-bit) and the readout speed.  The Neo has both a Rolling shutter mode and a Global shutter mode and the sensitivity of the 11-bit and 16-bit amplifiers is different for each mode.  The performance sheet of your Neo will contain the sensitivity values for each amplifier in both Rolling shutter and Global shutter.  

In would be happy to discuss this with you further, so please mail me ([log in to unmask]).

Best wishes,

Orla Hanrahan, PhD
Application Specialist, Life Science Imaging




-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Lu Lei
Sent: 26 July 2013 08:57
To: [log in to unmask]
Subject: Neo scMOS problem

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Hi all,
We recently purchased a wide-field microscope system with a scMOS camera (Neo, 5.5 Megapixel, by Andor). We are having quite some annoying problems with this Neo scMOS camera. Though it is a 16-bit device, it does not have the dynamic range it should have in our system.
1)	In "Rolling" shutter mode, the intensity value that the camera could 
generate is truncated at ~20K. In another word, the camera saturates at ~20K instead of 65535.
2)	In "Global" shutter mode, the camera saturates at ~57K-a little short of 
65535.
The camera is controled by the latest MetaMorph. The cooling temperature of the camera seems also normal as displayed by MetaMorph (-30&#61616;C). We haven't received any explanation from Andor via local vendor yet. I am wondering what is configured incorrectly here. 
Does anyone have similar experience of this camera or suggestion to share?
Thanks a lot.
Regards,
Lei Lu
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Lu Lei (Asst Prof) | School of Biological Sciences | Nanyang Technological University SBS-03n-18, 60 Nanyang Drive, Singapore 637551
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