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July 2013

CONFOCALMICROSCOPY@LISTS.UMN.EDU

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From:
Tamara Howard <[log in to unmask]>
Reply To:
Confocal Microscopy List <[log in to unmask]>
Date:
Fri, 12 Jul 2013 15:21:15 +0000
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We've used DyLight 649 - works beautifully on our SP5. 

Tamara
...................................................
Tamara Howard
Dept. of Cell Biology & Physiology
University of New Mexico
Albuquerque, NM


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From: Confocal Microscopy List [[log in to unmask]] on behalf of Andrew Lindsay [[log in to unmask]]
Sent: Friday, July 12, 2013 8:29 AM
To: [log in to unmask]
Subject: Fluorophores for 633nm laser

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Hi All,

I was wondering if people could recommend far-red fluorophores for
immunofluorescence that are excitable with a 633nm laser, I have tried
Alexa633 with little success. Have other people had more luck with secondary
antibodies conjugated to other fluorophores such as Cy5, DyLight649,
Alexa647.....?

Thanks in advance,
Andrew


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