Sender: |
|
Date: |
Mon, 12 Aug 2013 17:11:02 +0000 |
Reply-To: |
|
Subject: |
|
MIME-Version: |
1.0 |
Content-Transfer-Encoding: |
quoted-printable |
Content-Type: |
text/plain; charset="us-ascii" |
From: |
|
Comments: |
|
Parts/Attachments: |
|
|
*****
To join, leave or search the confocal microscopy listserv, go to:
http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Hi everyone:
I am having a weird problem with my Zeiss LSM 700 confocal microscope and hope you could shed light on it -
When I tried to scan with the lasers off (turning the lasers off via the ZEN software), I can literally see a relatively bright light coming from the objective! The light looks white, which I assume is the combination of the blue, green and red lasers. Although the detected fluorescence signal is 'minimal', it still worries that 1). It might photobleach my sample; and 2). When I scan with one laser, the other lasers might be on as well, so I wouldn't know what exactly I am scanning with!
I was told by Zeiss that this is "normal" because there are no mechanical shutters in the scan head. I have a hard time to believe it and here are my questions:
1). Does anyone experience the same/similar problem?
2). What could be the possible cause?
3). How am I supposed to fix it?
Thank you,
Zhaojie
Zhaojie Zhang, Ph. D.
Director, Jenkins Microscopy Facility
University of Wyoming
Laramie, WY 82071
PHONE: 307-766-3038
FAX: 307-766-5625
|
|
|