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Hi Zhaojie,
We had the same issue with our LSM700. The problem has been solved by our Zeiss engineer by tuning the "laser dark signal". This solved the problem for us.
As far as I know there is no AOTF inside the lsm700 and lasers are directly modulated.
Hope this helps.
All the best,
Pawel
________________________________________
Von: Confocal Microscopy List [[log in to unmask]]" im Auftrag von "G. Esteban Fernandez [[log in to unmask]]
Gesendet: Dienstag, 13. August 2013 06:12
An: [log in to unmask]
Betreff: Re: Zeiss LSM 700 Confocal- laser problem
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We've always seen that in our LSM 710 but the light is not from the lasers,
it is from the widefield excitation source (X-cite lamp). We can close the
lamp's shutter to get rid of the stray light. I've never seen it affect
the images but we close the shutter with dim specimens just to be safe.
-Esteban
On Mon, Aug 12, 2013 at 10:11 AM, Z.J. Zhang <[log in to unmask]> wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
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>
> Hi everyone:
>
> I am having a weird problem with my Zeiss LSM 700 confocal microscope and
> hope you could shed light on it -
>
> When I tried to scan with the lasers off (turning the lasers off via the
> ZEN software), I can literally see a relatively bright light coming from
> the objective! The light looks white, which I assume is the combination of
> the blue, green and red lasers. Although the detected fluorescence signal
> is 'minimal', it still worries that 1). It might photobleach my sample; and
> 2). When I scan with one laser, the other lasers might be on as well, so I
> wouldn't know what exactly I am scanning with!
>
> I was told by Zeiss that this is "normal" because there are no mechanical
> shutters in the scan head. I have a hard time to believe it and here are my
> questions:
>
> 1). Does anyone experience the same/similar problem?
> 2). What could be the possible cause?
> 3). How am I supposed to fix it?
>
> Thank you,
>
> Zhaojie
>
> Zhaojie Zhang, Ph. D.
> Director, Jenkins Microscopy Facility
> University of Wyoming
> Laramie, WY 82071
> PHONE: 307-766-3038
> FAX: 307-766-5625
>
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