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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Hi Alessandro,
I only managed to such stuff with live data mode-additional software that Leica sells.
But, i am not sure timing will be precise enough for you.... Better try it with a demo dongle
Good luck!
Katja
Sent from my HTC
----- Reply message -----
From: "Alessandro Esposito" <[log in to unmask]>
To: "[log in to unmask]" <[log in to unmask]>
Subject: Leica SP5 - adding delays between different positions in a multi point timelapse
Date: Wed, Aug 14, 2013 5:58 pm
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Dear all,
in order to synchronise the acquisition with external detectors, I would
need to add a delay after each position in a multi point time series using a
Leica SP5 confocal microscope.
I would like to create a list of positions with the mark and find tool. Then
setup a time lapse with the followins sequence:
1. move to next position (position 1 if start of loop)
2. acquire image
3. wait N seconds
4. loop to 1 until all position scanned
5. wait T minutes
6. loop to 1 until all time frames acquired
We have the last version of LAS AF compatible with the SP5. Do you know if
this is possible?
Cheers,
Alessandro
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