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Hi Doug,

Beta-catenin should work well for plasma membrane. Can also be detected 
in other parts of cells if certain signaling pathways were active.

Human Protein Atlas has data, and several (polyclonal) antisera. Most 
IHC vendors should have (monoclonal) antibodies to it.
http://www.proteinatlas.org/ENSG00000168036

*CTNNB1 <http://www.proteinatlas.org/ENSG00000168036>

*see, for example this tissue microarray core of a normal pancreas (can 
zoom up about 2x online)
http://www.proteinatlas.org/images/29159/62947_A_2_3.jpg

immunofluorescence of cells in culture are at
http://www.proteinatlas.org/ENSG00000168036/subcellular

Full credit to Prof. Vesa Kaartinen, who showed me awesome beta-catenin 
plasma membrane labeling of a tissue section (sorry, don't remember what 
body part, or species - probably mouse, maybe palate), when we were at 
Childrens Hospital Los Angeles. Vesa is now at U. Michigan School of 
Dentistry.

Enjoy,

George



On 8/14/2013 3:58 PM, Cromey, Douglas W - (dcromey) wrote:
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>
> I am working with a lab that has been trying to use Cadhedrin as a generic membrane marker for formalin-fixed, paraffin sectioned rat liver.  They are not having much luck.  Is there a good fixed tissue membrane marker that works reliably on paraffin sectioned tissue that you can recommend?
>
> Because of ongoing problems with autofluorescence, it would be highly preferable if the membrane fluorescence was not in the typical FITC/GFP emission range, we would highly prefer something more red-shifted.
>
> Doug
>
> ^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
> Douglas W. Cromey, M.S. - Associate Scientific Investigator
> Dept. of Cellular&  Molecular Medicine, University of Arizona
> 1501 N. Campbell Ave, Tucson, AZ  85724-5044 USA
>
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>    


-- 



George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
http://works.bepress.com/gmcnamara/26/