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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****
Laurent -
Calcofluor or other cellulose-binders are typical, caveat being I
don't work with yeasts.. A membrane stain called FUN has also been
used. See papers with Ghannoum in the author line.
Rob
On Aug 15, 2013, at 5:46 AM, Gelman, Laurent wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Hello everybody,
>
>
> We would need to stain living S. Pombe cells imaged on a
> fluorescence microscope to segment thereafter during image analysis
> automatically the cells.
> Staining cell wall and/or membrane would be perfect. Has someone any
> specific experience with yeast and/or Pombe?
>
> Note that we need an organic dye and cannot express any recombinant
> protein (we are screening a yeast library).
>
> Thanks for your advices!
>
> Best regards,
>
>
> Laurent.
>
>
>
> _____________________________________________
> Laurent Gelman, PhD
> Head of Facility for Advanced Imaging and Microscopy
> (Light Microscopy)
>
> Friedrich Miescher Institut
> WRO 1066.2.16
> Maulbeerstrasse 66
> CH-4058 Basel
> +41 (0)61 696 43 38
> +41 (0)79 618 73 69
Robert J. Palmer Jr., Ph.D.
Microbial Receptors Section
Laboratory of Cell and Developmental Biology
Natl Inst Dental Craniofacial Res - Natl Insts Health
Bldg 30, Room 207
30 Convent Drive
Bethesda MD 20892
ph 301-594-0025
fax 301-402-0396
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