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Nicola, 

When you export your image from the 510 as a TIFF file, the software should give you the option to add a scale bar to the image. Ask a Zeiss rep if you don't find it. Alternately, your lab may own or borrow a graticule (also referred to as reticule or stage micrometer), i.e. a glass slide engraved with a 1 or 2mm scale, with marks for 10 and 100 microns intervals. Take a transmitted light image of the scale using each of the lenses present on the confocal and with a set zoom factor (important). You can then store these images and go back to the appropriate one to create a scale bar for any image, past or future. Just don't forget to take the zoom factor into account.

Evelyn Ralston, Ph.D.
Light Imaging Section
NIAMS, National Institutes of Health,
Bethesda, MD 20892





On Aug 16, 2013, at 6:56 AM, Nicola Green wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
> 
> Hi
> This is probably a very foolish and basic question, but I am going to ask
> it any way and just apologise for my ignorance as a biological specialist
> using a confocal microscope.
> 
> In microscopy I would generally multiply the objective lens magnification
> by the ocular lens (often 10x) to get the total magnification of an image.
> I want to know if this also applies for the confocal microscope or is the
> light path such that the objective magnification is the only one relevant?
> If I do need to include additional magnification what would these
> magnifications be, are they dependent upon the system being used (LSM 510
> META)  or is there a standard magnification?
> 
> Thanks for your help in clarifying this for me.
> 
> Regards
> Nicola