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Andrew,
I do not think that there can ever be a "gold standard". As there are
components needed within the reconstruction that are essentially ill-posed,
a multitude of algorithmic solutions approaching the same problem, can be
utilized. While other methods that were not originally considered by Mats
Gustafsson, such as reconstruction in space domain (e.g. Stallinga et. al)
give algorithmic advantages within some components, the original reciprocal
space methods can't, it is hard to create a general processing pipeline that
take advantage of all variants. However, you might read from time to time
about advances on critical aspects, such as "Structured illumination
microscopy: artefact analysis and reduction utilizing a parameter
optimization approach", (L.H. Schaefer et. al, 2004) or more recently:
"Phase Optimization for structured illumination microscopy" (Kay Wicker et.
al, 2013).
For these reasons, you will find that almost everyone who wants to process
SIM data in a research context will do it on their own. There are just too
many optimization variables, forbidding a general "gold standard" solution.
For Mats classical academic implementations (Matlab, etc.) that you mention,
I am sure you can ask several authors for the code, as long as they are not
exclusively affiliated with commercial companies.
Regards
Lutz
__________________________________
L u t z S c h a e f e r
Sen. Scientist
Mathematical modeling / Computational microscopy
Advanced Imaging Methodology Consultation
16-715 Doon Village Rd.
Kitchener, ON, N2P 2A2, Canada
Phone/Fax: +1 519 894 8870
Mobile: +1 519 722 8870
Email: [log in to unmask]
Website: http://home.golden.net/~lschafer/
___________________________________
-----Original Message-----
From: Andrew York
Sent: Monday, August 12, 2013 2:27 AM
To: [log in to unmask]
Subject: Is Gustafsson-style SIM processing code available?
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In the papers I've read, Gustafsson et al. give excellent descriptions of
their processing algorithms, but I haven't stumbled on any actual
processing code, source or compiled, which turns raw SIM data into
superresolution images. I've rolled my own just for fun, but it would be
nice to compare to the 'gold standard'. Is this code available somewhere
that I'm missing? If not, is someone working on this, or does everyone who
builds a SIM just roll their own?
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