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This is a very LATE reply, but I would suggest that you consider 
upgrading to a camera with no fan.  Or, if you don't mind voiding the 
warranty strip the case off the camera and use an external (not 
mechanically coupled to the camera) fan to cool it.

The bottom line is that fan will almost always start to vibrate at some 
point!

Chris Tully
Image Analysis Expert
www.ImageIncyte.com
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On 4/24/2013 12:39 PM, Pedro Almada wrote:
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>
> Dear Listers, especially to all those who contacted me off-list with
> suggestions,
>
> After much headscratching and some visits from Olympus and Image Solutions,
> we've finally pinpointed the problem. It seems the fan of the camera was
> introducing a vibration that was not detectable until we could upgrade the
> software to be able to expose for under 35ms. With 1ms exposures, we saw
> that the double beads were actually single beads vibrating at a fairly
> consistent direction and distance, which averaged to a "double" psf on both
> the eyepiece and camera!
>
> After further headstratching and turning everything off step by step we saw
> it was the fan of the camera (!) that was introducing the vibrations! It
> should be a matter of replacing that now.
>
> Anyway, I'd like to thank all those who offered help and I hope this story
> will actually be of use to someone in the future.
>
> Thank you,
> Pedro Almada
>
>
> On 2 April 2013 18:53, Pedro Almada <[log in to unmask]> wrote:
>
>> Dear listers,
>>
>> This is not strictly a confocal question since it's about a widefield
>> system, but I think it's quite suited to the readers of this list.
>>
>> Our users of a DeltaVision system (an Olympus IX71) were complaining of
>> "blurry" images. After checking that their parameters were normal we made a
>> stack of 0.17 um beads and observed that the PSF was very wrong: we could
>> see two spots instead of one! It's not the DIC slider (the obvious culprit)
>> and it's independent of what dichroic or emission filters we use. The
>> problem is present in all objectives but gets worse with magnification.
>> With the 40x we just see elongated spots but with the 100x we clearly see
>> two spots. This was not there before.
>>
>> You can observe all of this by yourselves in the files available on this
>> folder. I've included stacks and comparison shots of PSF's acquired in
>> December.
>> https://www.dropbox.com/sh/i770v4a0a4owrb8/p_qBYW4E-2
>>
>> Do anyone have a clue on what could be causing this?
>>
>> Preemptive thanks for your input,
>> Best regards,
>> Pedro Almada
>>