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November 2013

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Wed, 6 Nov 2013 01:48:13 +0000
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How much of the 70 mW is from UV or IR leaking through?
_________________________________________
Michael Cammer, Assistant Research Scientist
Skirball Institute of Biomolecular Medicine
Lab: (212) 263-3208  Cell: (914) 309-3270

________________________________________
From: Confocal Microscopy List [[log in to unmask]] on behalf of Julio Vazquez [[log in to unmask]]
Sent: Tuesday, November 05, 2013 5:09 PM
To: [log in to unmask]
Subject: Re: Brightness difference Hg vs LED


A quick comparison in our lab gave the following:

488 nm (FITC) band of an LED-based light engine (Lumencor): 40 mW
300 W Xenon Sutter illuminator (probably our brightest light source) with FITC EX filter: 70 mW

both measured at the exit of the fiber or light guide.

I agree that LED based light engines are probably at least as good (power-wise) as most lamps, plus they have other benefits. Charts such as the one found here should be a good guide regarding relative power:

http://lumencor.com/wp-content/uploads/2011/09/SpectraX-LE-User-Manual1.pdf


Julio Vazquez, PhD
Fred Hutchinson Cancer Research Center
Seattle, WA 98109

http://www.fhcrc.org/en.html


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