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These papers might be helpful.

Comparing systems:
Evaluating performance in three-dimensional fluorescence microscopy
JOHN M MURRAY, PAUL L APPLETON, JASON R SWEDLOW, JENNIFER C WATERS
J Microsc. 2007 December; 228(3): 390–405. doi:
10.1111/j.1365-2818.2007.01861.x
PMCID:     PMC2438600

Calibration, theory etc.:
Precise nanometer localization analysis for individual fluorescent probes.
Russell E Thompson, Daniel R Larson, Watt W Webb
Biophys J. 2002 May; 82(5): 2775–2783.
PMCID:     PMC1302065

_____________________________________
Philippe Laissue, PhD, Bioimaging Manager
School of Biological Sciences, Room 4.17
University of Essex, Colchester CO4 3SQ, UK
(0044) 01206 872246 / (0044) 07842 676 456
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privatewww.essex.ac.uk/~plaissue


On 7 November 2013 12:33, Csúcs Gábor <[log in to unmask]> wrote:

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> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Dear All,
>
> I made a quick search but found nothing really useful. My question is the
> following: does anyone have some data/literature how efficient microscopes
> are in detecting the photons of a fluorescent sample? Obviously the NA and
> the QE of the camera (plus the performance filters/dichroics) are
> important factors but taken e.g. A single molecule experiment with a 1.49
> NA objective and a back-illuminated EM-CCD what ration of the emitted
> photons really reach the detector? Of course the transmission of the
> objectives and other lenses in the microscopes can vary but is there still
> an estimate?
>
> Thanks     Gabor
>