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Oliver,

I would highly recommend asking around among your local colleagues for 
someone who has a fluorescence spectrophotometer, and measure the curves 
yourself.

Alternatively, I have read this article because I do not have access to 
it at the moment but according to Google it does have a UV-Vis 
absorbance spectrum.

Chris

On 11/5/2013 10:45 AM, Oliver Biehlmaier wrote:
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> To join, leave or search the confocal microscopy listserv, go to:
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>
> Dear listers,
>
> I have a question regarding Evans Blue:
>
> I have a user that is doing standard 3ch-labelings on a confocal microscope (DAPI, Alexa488, Alexa 568). Now she is having some sections from mice that have been injected with Evans Blue.
> When imaging these new samples, she wants to use the same 3ch approach as before but she does not want to see any fluorescence from the Evans Blue.
> I tried to find out what the excitation and emission spectrum of Evans Blue is but I could not find any clear information. E.g. one publication specifies the spectrum of Evans Blue as follows: excitation at 620 nm, emission at 680 nm (Saria A, Lundberg JM. Evans blue fluorescence: quantitative and morphological evaluation of vascular permeability in animal tissues. J Neurosci Methods. 1983 May;8(1):41-9. PubMed PMID: 6876872.) Others claim that it fluoresces with excitation peaks at 470 and 540 nm and that it has its emission peak at 680 nm. I tend towards the first spectrum but I did not find any other confirmation for it.
>
> Does anyone of you guys know the exact spectrum of that dye?
> This would be great!
>
> Cheers,
> Oliver
>
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