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November 2013

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Confocal Microscopy List <[log in to unmask]>
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Mon, 18 Nov 2013 11:47:01 +0100
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Re: 2P vs 1P psf
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Steffen Dietzel <[log in to unmask]>
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*****
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http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
*****

Citable literature is sparse in that area. For two-photon and confocal 
resolution formula I usually cite these two, the first one being the one 
that Guy already mentioned:

G. Cox, C. J. Sheppard: Practical limits of resolution in confocal and 
non-linear microscopy. In: Microscopy Research and Technique. Band 63, 
Issue 1, 2004, p. 18–22, doi:10.1002/jemt.10423, PMID 14677129.

B. Amos, G. McConnell, T. Wilson: Confocal microscopy. In: E. Egelman 
(Ed.): Biophysical Techniques for Characterization of Cells (= 
Comprehensive Biophysics. 2). Elsevier, Academic Press, Amsterdam 2012, 
ISBN 978-0-12-374920-8, 2, p. 3–23, doi:10.1016/B978-0-12-374920-8.00203-4
Author version:
http://www2.mrc-lmb.cam.ac.uk/images/groupleaders/Confocal_microscopy_Amos_McConnell_Wilson.pdf


When it comes to three-photon excition it gets really thin. I just very 
recently came accross a paper which gives the xy-resolution formula 
(0.51*lambda)/(sqrt(3)*NA), see below. If anyone should have an older 
citable reference. I very much would like to hear about it.

Steffen

Jung Ho Yu, Seung-Hae Kwon, Zdenˇek Petrášek3, Ok Kyu Park, Samuel 
Woojoo Jun, Kwangsoo Shin, Moonkee Choi, Yong Il Park, Kyeongsoon Park, 
Hyon Bin Na, Nohyun Lee, Dong Won Lee, Jeong Hyun Kim, Petra Schwille, 
Taeghwan Hyeon: High-resolution three-photon biomedical imaging using 
doped ZnS nanocrystals. In: Nature Materials. 12, 2013, p. 359–366, 
doi:10.1038/NMAT3565

On 17.11.2013 16:06, Jeff Reece wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Is there a reference for the confocal psf being equal to the excitation psf when the pinhole=1AU (free access via the web is requested).  It is not intuitive that the confocal psf would leave out the optics (pinhole) of the emission path.
>
> Thanks,
> Jeff
>


-- 
------------------------------------------------------------
Steffen Dietzel, PD Dr. rer. nat
Ludwig-Maximilians-Universität München
Walter-Brendel-Zentrum für experimentelle Medizin (WBex)
Head of light microscopy

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