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3-photon confocal vs 3-photon PSF vs other modes (calculations) in
figure 2 of Schrader, Bahlmann, Hell 1997 Optik
http://www3.mpibpc.mpg.de/groups/hell/publications/pdf/Optik_104_116-124.pdf
Here is a recent paper on tri-exciton (a 3 photon process) imaging (see
references for earlier triexciton imaging):
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0064023
his study describes a simple technique that improves a recently
developed 3D sub-diffraction imaging method based on three-photon
absorption of commercially available quantum dots. The method combines
imaging of biological samples via tri-exciton generation in quantum dots
with deconvolution and spectral multiplexing, resulting in a novel
approach for multi-color imaging of even thick biological samples at a
1.4 to 1.9-fold better spatial resolution. This approach is realized on
a conventional confocal microscope equipped with standard
continuous-wave lasers. We demonstrate the potential of multi-color
tri-exciton imaging of quantum dots combined with deconvolution on viral
vesicles in lentivirally transduced cells as well as intermediate
filaments in three-dimensional clusters of mouse-derived neural stem
cells (neurospheres) and dense microtubuli arrays in myotubes formed by
stacks of differentiated C2C12 myoblasts.
enjoy,
George
On 11/18/2013 4:47 AM, Steffen Dietzel wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> Citable literature is sparse in that area. For two-photon and confocal
> resolution formula I usually cite these two, the first one being the
> one that Guy already mentioned:
>
> G. Cox, C. J. Sheppard: Practical limits of resolution in confocal and
> non-linear microscopy. In: Microscopy Research and Technique. Band 63,
> Issue 1, 2004, p. 18–22, doi:10.1002/jemt.10423, PMID 14677129.
>
> B. Amos, G. McConnell, T. Wilson: Confocal microscopy. In: E. Egelman
> (Ed.): Biophysical Techniques for Characterization of Cells (=
> Comprehensive Biophysics. 2). Elsevier, Academic Press, Amsterdam
> 2012, ISBN 978-0-12-374920-8, 2, p. 3–23,
> doi:10.1016/B978-0-12-374920-8.00203-4
> Author version:
> http://www2.mrc-lmb.cam.ac.uk/images/groupleaders/Confocal_microscopy_Amos_McConnell_Wilson.pdf
>
>
>
> When it comes to three-photon excition it gets really thin. I just
> very recently came accross a paper which gives the xy-resolution
> formula (0.51*lambda)/(sqrt(3)*NA), see below. If anyone should have
> an older citable reference. I very much would like to hear about it.
>
> Steffen
>
> Jung Ho Yu, Seung-Hae Kwon, Zdenˇek Petrášek3, Ok Kyu Park, Samuel
> Woojoo Jun, Kwangsoo Shin, Moonkee Choi, Yong Il Park, Kyeongsoon
> Park, Hyon Bin Na, Nohyun Lee, Dong Won Lee, Jeong Hyun Kim, Petra
> Schwille, Taeghwan Hyeon: High-resolution three-photon biomedical
> imaging using doped ZnS nanocrystals. In: Nature Materials. 12, 2013,
> p. 359–366, doi:10.1038/NMAT3565
>
> On 17.11.2013 16:06, Jeff Reece wrote:
>> *****
>> To join, leave or search the confocal microscopy listserv, go to:
>> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
>> *****
>>
>> Is there a reference for the confocal psf being equal to the
>> excitation psf when the pinhole=1AU (free access via the web is
>> requested). It is not intuitive that the confocal psf would leave
>> out the optics (pinhole) of the emission path.
>>
>> Thanks,
>> Jeff
>>
>
>
--
George McNamara, Ph.D.
Single Cells Analyst
L.J.N. Cooper Lab
University of Texas M.D. Anderson Cancer Center
Houston, TX 77054
Tattletales http://works.bepress.com/gmcnamara/26/
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