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Hi Phil I would say that the new LED sources and Light Engines will be brighter than mercury sources. For example I compared an Exfo to a SOLA light engine and the light engine is brighter. The new Lumen Dynamics XLED should provide much better illumination than a mercury source.
Hopefully, mercury sources will soon be a thing of the past.
Cheers,
Brian D Armstrong PhD
Associate Research Professor
Director, Light Microscopy Core
Beckman Research Institute
City of Hope
Dept of Neuroscience
1450 E Duarte Rd
Duarte, CA 91010
626-256-4673 x62872
-----Original Message-----
From: Confocal Microscopy List [mailto:[log in to unmask]] On Behalf Of Philip Oshel
Sent: Tuesday, November 05, 2013 9:57 AM
To: [log in to unmask]
Subject: Brightness difference Hg vs LED
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All,
I had this question put to me by a new faculty member, and don't have a
ready answer:
"Is there a ballpark percentage for how much less bright an LED vs a
standard mercury lamp light?"
This is for regular epifluorescence, not confocal.
This is in the realm of arm-waving over a picture of beer (a good, dark
stout), ignoring brands, how old the Hg bulb is, ex/em cubes, which part
of the spectrum is used, and all that. Personally, I'd think the answer
is more like, "Doesn't matter, the dimmer system is still too bright to
use all the available light and not damage the specimen." But ... ?
Phil
--
Philip Oshel
Microscopy Facility Supervisor
Biology Department
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
(989) 774-3576
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