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November 2013

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Fri, 22 Nov 2013 11:54:31 +0000
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Confocal Microscopy List <[log in to unmask]>
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Re: Staining Plant roots for actin
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Check out David Collings' papers. I think this one has intact root double labelling of actin and tubulin:

Collings, D.A. and Wasteneys, G.O. (2005) Actin microfilament and microtubule distribution patterns in the expanding root of Arabidopsis thaliana. Canadian Journal of Botany 83: 579-590
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From: Confocal Microscopy List [[log in to unmask]] on behalf of Terri Bruce [[log in to unmask]]
Sent: Friday, 22 November 2013 4:41 p.m.
To: [log in to unmask]
Subject: Staining Plant roots for actin

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Hi!

Does anyone have any protocols for fixing and staining actin in whole plant roots that works really well?

All suggestions are appreciated!

Thanks!
Terri

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