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November 2013

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Date:
Tue, 5 Nov 2013 18:30:28 +0000
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Confocal Microscopy List <[log in to unmask]>
Subject:
Re: Brightness difference Hg vs LED
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"Doube, Michael" <[log in to unmask]>
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*****
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Hi Phil,

no idea about percentages but in my hands a single blue high power LED
like this one, is 'enough' for practical application, when set directly
in place of a 50W Hg lamp, on a heatsink in the lamphouse:

http://uk.rs-online.com/web/p/visible-leds/7393548/  (no commercial
interest)

Provided of course that you only want blueish excitation...

You can also run them off very simple and cheap switching boxes, if you
are not afraid of a soldering iron.

Michael

On 05/11/13 18:00, Philip Oshel wrote:
> *****
> To join, leave or search the confocal microscopy listserv, go to:
> http://lists.umn.edu/cgi-bin/wa?A0=confocalmicroscopy
> *****
>
> All,
>
> I had this question put to me by a new faculty member, and don't have a
> ready answer:
> "Is there a ballpark percentage for how much less bright an LED vs a
> standard mercury lamp light?"
> This is for regular epifluorescence, not confocal.
>
> This is in the realm of arm-waving over a picture of beer (a good, dark
> stout), ignoring brands, how old the Hg bulb is, ex/em cubes, which part
> of the spectrum is used, and all that. Personally, I'd think the answer
> is more like, "Doesn't matter, the dimmer system is still too bright to
> use all the available light and not damage the specimen." But ... ?
>
> Phil


--
Dr Michael Doube
BPhil BVSc PhD MRCVS
Lecturer, Comparative Biomedical Sciences
The Royal Veterinary College
London NW1 0TU
United Kingdom

+44 (0)20 7121 1903

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